NEETBotanyPlant - Growth and Development
What did F. Skoog and his co-workers observe in tobacco stem internodal segments?
Options
- AProliferation of leaves
- BCallus proliferation only with auxins
- CCallus proliferation with auxins and specific supplements
- DNo proliferation
Correct answer
C. Callus proliferation with auxins and specific supplements
Step-by-step solution
F. Skoog and his co-workers observed that from the internodal segments of tobacco stems the callus (a mass of undifferentiated cells) proliferated only if, in addition to auxins the nutrients medium was supplemented with one of the following: extracts of vascular tissues, yeast extract, coconut milk or DNA. Skoog and Miller, later identified and crystallised the cytokinesis promoting active substance that they termed kinetin.