What are the other factors that can interfere with the binding of O₂ with haemoglobin?

Step-by-step solution

. Haemoglobin is a red coloured iron containing pigment present in the RBCs. O2 can bind with haemoglobin in a reversible manner to form oxyhaemoglobin. Each haemoglobin molecule can carry a maximum of four molecules of O2. Binding of oxygen with haemoglobin is primarily related to partial pressure of O2. Partial pressure of CO2, hydrogen ion concentration and temperature are the other factors which can interfere with this binding . A sigmoid curve is obtained when percentage saturation of haemoglobin with O2 is pl