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MHT CET 202624 AprilMorning Shift
Exam
MHT CET
Year
2026
Date
24 April
Shift
Morning Shift
Subject
Biology
Chapter
Mineral Nutrition

MHT CET 2026 · 24 April · Morning Shift · Biology · Mineral Nutrition

Which among the following represents amidation during nitrogen...

Which among the following represents amidation during nitrogen fixation in plants?

Options

  1. Aα\alpha-Ketoglutaric acid +NH4+NADPH2→+\text{NH}_4+\text{NADPH}_2\rightarrow Glutamic acid +H2O++\text{H}_2\text{O}+ NADP
  2. BGlutamic acid ++ oxaloacetic acid →α\rightarrow\alpha-Ketoglutaric acid ++ Aspartic acid
  3. CGlutamic acid +NH4++\text{NH}_4+ ATP →α\rightarrow\alpha-glutamine ++ ADP
  4. D2NH3+3O2→2HNO2+2H2O2\text{NH}_3+3\text{O}_2\rightarrow2\text{HNO}_2+2\text{H}_2\text{O}

Correct answer

C. Glutamic acid +NH4++\text{NH}_4+ ATP →α\rightarrow\alpha-glutamine ++ ADP

Step-by-step solution

Amidation is the process in which an amino acid reacts with an ammonium ion to form an amide. This process requires energy in the form of ATP.

In plants, the hydroxyl part of the acid group of an amino acid is replaced by another amino radical (extNH2−ext{NH}_2^-). For example, glutamic acid reacts with extNH4+ext{NH}_4^+ in the presence of ATP to form glutamine.

The reaction is:
Glutamic acid +extNH4+++ ext{NH}_4^++ ATP → Glutamine ++ ADP ++ Pi

The first option represents reductive amination.
The second option represents transamination.
The fourth option represents nitrification.

Answer: Glutamic acid +NH4++\text{NH}_4+ ATP →α\rightarrow\alpha-glutamine ++ ADP
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