Given below are two statements : one is labelled as Assertion A and the other is labelled as Reason R . Assertion A : In recombinant DNA technology, lysozyme is used for disrupting bacterial cells while cellulase is for plant cells. Reason R : Isolation of genetic material needs disruption of cells. In the light of the above statements, choose the most appropriate answer from the options given below :
C. Both A and R are correct but R is not the correct explanation of A
Assertion A is a correct statement. In recombinant DNA technology, bacterial cells are treated with the enzyme lysozyme to degrade their peptidoglycan cell walls, while plant cells are treated with cellulase to degrade their cellulosic cell walls. Reason R is also a correct statement. The isolation of genetic material requires the disruption of the cell to release DNA along with other macromolecules like RNA, proteins, polysaccharides, and lipids. However, Reason R does not explain the specificity of the enzymes mentioned in Assertion A. The correct explanation for Assertion A would be the difference in the biochemical composition of the cell walls of bacteria and plants. Therefore, both Assertion A and Reason R are correct, but Reason R is not the correct explanation of Assertion A.
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