Arrange the following steps of DNA fingerprinting in a correct sequence. A. Isolation of DNA and its digestion by restriction endonucleases. B. Hybridisation using a labelled VNTR probe. C. Transferring of separated DNA fragments to synthetic membranes. D. Detection of hybridised DNA fragments by autoradiography. E. Separation of DNA fragments by electrophoresis. Choose the correct answer from the options given below :
C. A, E, C, B, D
The technique of DNA fingerprinting involves the following steps in sequence: 1. Isolation of DNA and its digestion by restriction endonucleases (A). 2. Separation of the digested DNA fragments by gel electrophoresis (E). 3. Transferring (blotting) of the separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon (C). 4. Hybridisation using a radiolabelled VNTR probe (B). 5. Detection of the hybridised DNA fragments by autoradiography (D). The correct sequence is A, E, C, B, D. Answer: A, E, C, B, D
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