Arrange the following steps of somatic hybridisation in a correct sequence. A. Digestion of cell walls. B. Isolation of naked protoplasts. C. Fusion of protoplasts to get hybrid protoplast. D. Isolation of single cells from two different varieties of plants. E. Growing of hybrid protoplast to form a new plant. Choose the correct answer from the options given below :
A. D, A, B, C, E
Somatic hybridisation involves the fusion of protoplasts of two different plant varieties to produce a hybrid plant. The correct sequence of steps is as follows: First, single cells are isolated from two different varieties of plants (Step D). Next, the cell walls of these isolated cells are digested using enzymes such as cellulase and pectinase (Step A). This digestion results in the isolation of naked protoplasts, which are cells without cell walls (Step B). The isolated naked protoplasts from the two different varieties are then fused together, often using chemicals like PEG or electrical stimulation, to get a hybrid protoplast (Step C). Finally, the hybrid protoplast is cultured and grown to form a new plant (Step E). Thus, the correct sequence is D, A, B, C, E. Answer: D, A, B, C, E
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