Protein found in a biological system with a unique three-dimensional structure and biological activity is called a native protein. When a protein in its native form, is subjected to a physical change like change in temperature or a chemical change like, change in pH, denaturation of protein takes place. Explain the cause.

Options

1. AChanges in temperature or pH disrupt the covalent bonds holding the protein's primary structure together.
2. BDue to physical or chemical change, hydrogen bonds in proteins are disturbed, globules unfold and the helix ge
3. CThe native protein undergoes a chemical reaction with the environment, leading to structural changes.
4. DDenaturation is a result of protein degradation caused by enzymes.