What must be regenerated from NADH and FADH2 for the TCA cycle to...

During the conversion of succinyl-CoA to succinic acid a molecule of GTP is synthesised. This is a substrate level phosphorylation. In a coupled reaction GTP is converted to GDP with the simultaneous synthesis of ATP from ADP. Also there are three points in the cycle where NAD+ is reduced to NADH + H⁺ and one point where FAD+ is reduced to FADH2 . The continued oxidation of acetyl CoA via the TCA cycle requires the continued replenishment of oxaloacetic acid, the first member of the cycle. In addition it also requires regeneration of NAD+ and FAD+ from NADH and FADH2 respectively.