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In a standard graph of the velocity of an enzyme-catalysed reaction (y-axis) against substrate concentration (x-axis), the curve obtained is a rectangular hyperbola. The horizontal asymptote of this curve is denoted by 'P'. The value on the y-axis corresponding to exactly half of 'P' is denoted by 'Q'. The corresponding value on the x-axis that yields a velocity of 'Q' is denoted by 'R'. Identify the kinetic paramete

Options

  1. AP = K_ m , Q = V_ max 2 , R = V_ max
  2. BP = V_ max , Q = K_ m , R = V_ max 2
  3. CP = V_ max 2 , Q = V_ max , R = K_ m
  4. DP = V_ max , Q = V_ max 2 , R = K_ m

Correct answer

D. P = V_ max , Q = V_ max 2 , R = K_ m

Step-by-step solution

In the graph of enzyme reaction velocity (v) versus substrate concentration (S), the curve is a rectangular hyperbola. The velocity reaches a maximum limit which is represented by the horizontal asymptote. Thus, P represents the maximum velocity, V_ max . Q is exactly half of this maximum velocity on the y-axis, so Q represents V_ max 2 . The substrate concentration on the x-axis at which the reaction velocity is half of its maximum value ( V_ max 2 ) is known as the Michaelis constant ( K_ m ). Therefore, R repres

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