Match List-I with List-II regarding enzyme kinetics. List-I List-II A. V_ max I. Velocity rises steeply with increase in substrate concentration B. K_m II. Substrate concentration at half-maximal velocity C. Initial phase III. Maximum velocity achieved by the reaction D. Saturation phase IV. All enzyme active sites are occupied and no free enzyme is available Choose the correct answer from the options given below:

Step-by-step solution

V_ max represents the maximum velocity achieved by the reaction when all enzyme active sites are saturated. K_m (Michaelis constant) is defined as the substrate concentration at which the reaction velocity is half of V_ max . During the initial phase of the reaction, the velocity rises steeply as substrate concentration increases. In the saturation phase, the reaction velocity does not increase further because all enzyme active sites are occupied by substrate molecules and no free enzyme is available. Therefore, th