NEETZoologyMolecular Basis of Inheritance
What is the main function of the enzyme DNA-dependent DNA polymerase in the process of replication in living cells like E. coli?
Options
- ATo separate the two strands of DNA
- BTo catalyze the polymerization of deoxynucleotides
- CTo make mistakes during replication
- DTo act as a template for the synthesis of new complementary strands
Correct answer
B. To catalyze the polymerization of deoxynucleotides
Step-by-step solution
In living cells, such as E. coli, the process of replication requires a set of catalysts (enzymes). The main enzyme is referred to as DNA-dependent DNA polymerase, since it uses a DNA template to catalyze the polymerisation of deoxynucleotides. These enzymes are highly efficient enzymes as they have to catalyze polymerisation of a large number of nucleotides in a very short time. E. coli that has only 4.6 ×106 bp (compare it with humans whose diploid content is 6.6 × 109 bp), completes the process of replication wi