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NEETZoologyMolecular Basis of Inheritance

What is the direction of polymerization catalyzed by DNA-dependent DNA polymerases?

Options

  1. A3' to 5'
  2. B5' to 3'
  3. Cboth 3' to 5' and 5' to 3'
  4. Dnone of the above

Correct answer

B. 5' to 3'

Step-by-step solution

For long DNA molecules, since the two strands of DNA cannot be separated in its entire length (due to very high energy requirement), the replication occurs within a small opening of the DNA helix, referred to as replication fork. The DNA-dependent DNA polymerases catalyze polymerisation only in one direction, that is 5' 🡪 3' . This creates some additional complications at the replication fork. Consequently, on one strand (the template with polarity 3'🡪5'), the replication is continuous, while on the other (the temp

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