NEETZoologyMolecular Basis of Inheritance
What is the purpose of vectors in recombinant DNA procedures?
Options
- Ato provide the origin of replication
- Bto catalyze polymerization
- Cto join discontinuously synthesized fragments
- Dnone of the above
Correct answer
A. to provide the origin of replication
Step-by-step solution
The DNA polymerases on their own cannot initiate the process of replication. Also the replication does not initiate randomly at any place in DNA. There is a definite region in E. coli DNA where the replication originates. Such regions are termed as origin of replication. It is because of the requirement of the origin of replication that a piece of DNA, if needed to be propagated during recombinant DNA procedures, requires a vector. The vectors provide the origin of replication. Further, not every detail of replicat