NEETZoologyMolecular Basis of Inheritance
What is the purpose of density gradient centrifugation in DNA fingerprinting?
Options
- ATo separate repetitive DNA from bulk genomic DNA
- BTo identify polymorphic regions in DNA sequence
- CTo compare DNA sequences of two individuals
- DTo form a major peak of satellite DNA
Correct answer
A. To separate repetitive DNA from bulk genomic DNA
Step-by-step solution
DNA fingerprinting involves identifying differences in some specific regions in DNA sequence called repetitive DNA, because in these sequences, a small stretch of DNA is repeated many times. These repetitive DNA are separated from bulk genomic DNA as different peaks during density gradient centrifugation . The bulk DNA forms a major peak and the other small peaks are referred to as satellite DNA. Depending on base composition (A : T rich or G:C rich), length of segment, and number of repetitive units, the satellite