NEETZoologyMolecular Basis of Inheritance
What is the purpose of using synthetic membranes in DNA fingerprinting?
Options
- ATo isolate DNA
- BTo digest DNA by restriction endonucleases
- CTo separate DNA fragments by electrophoresis
- DTo transfer separated DNA fragments for hybridisation
Correct answer
D. To transfer separated DNA fragments for hybridisation
Step-by-step solution
The technique of DNA Fingerprinting was initially developed by Alec Jeffreys. He used a satellite DNA as a probe that showed a very high degree of polymorphism. It was called the Variable Number of Tandem Repeats (VNTR). The technique, as used earlier, involved Southern blot hybridisation using radiolabeled VNTR as a probe. It included (i) isolation of DNA, (ii) digestion of DNA by restriction endonucleases, (iii) separation of DNA fragments by electrophoresis, (iv ) transferring (blotting) of separated DNA fragmen