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NEETZoologyMolecular Basis of Inheritance

What is the purpose of using synthetic membranes in DNA fingerprinting?

Options

  1. ATo isolate DNA
  2. BTo digest DNA by restriction endonucleases
  3. CTo separate DNA fragments by electrophoresis
  4. DTo transfer separated DNA fragments for hybridisation

Correct answer

D. To transfer separated DNA fragments for hybridisation

Step-by-step solution

The technique of DNA Fingerprinting was initially developed by Alec Jeffreys. He used a satellite DNA as a probe that showed a very high degree of polymorphism. It was called the Variable Number of Tandem Repeats (VNTR). The technique, as used earlier, involved Southern blot hybridisation using radiolabeled VNTR as a probe. It included (i) isolation of DNA, (ii) digestion of DNA by restriction endonucleases, (iii) separation of DNA fragments by electrophoresis, (iv ) transferring (blotting) of separated DNA fragmen

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