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NEETZoologyMolecular Basis of Inheritance

Arrange the following fundamental steps of DNA fingerprinting in the correct chronological order: A. Digestion of DNA by restriction endonucleases B. Separation of DNA fragments by gel electrophoresis C. Hybridisation using a radiolabelled VNTR probe D. Transferring of separated DNA fragments to a synthetic membrane E. Detection of hybridised DNA fragments by autoradiography Choose the correct answer from the options

Options

  1. AA B D C E
  2. BA B C D E
  3. CB A D C E
  4. DA D B E C

Correct answer

A. A B D C E

Step-by-step solution

Refer: NCERT | Class 12 | Page: 106 | Paragraph: 2 The fundamental steps of DNA fingerprinting are carried out in the following sequence: 1. Isolation of DNA. 2. Digestion of DNA by restriction endonucleases (A). 3. Separation of DNA fragments by gel electrophoresis (B). 4. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon (D). 5. Hybridisation using a radiolabelled VNTR probe (C). 6. Detection of hybridised DNA fragments by autoradiography (E). Thus, the cor

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