A researcher aims to profile only the transcriptionally active genes of a newly discovered eukaryotic genome, deliberately ignoring all unexpressed sequences and non-coding DNA. Which of the following methodologies used during the Human Genome Project is most appropriate for this goal?

Step-by-step solution

During the Human Genome Project, two major methodologies were used. The approach that focuses exclusively on identifying all the genes that are expressed as RNA (transcriptionally active) is called Expressed Sequence Tags (ESTs). This method effectively ignores unexpressed and non-coding DNA. In contrast, Sequence Annotation involves taking a blind approach to sequence the entire genome (both coding and non-coding regions) and later assigning functions to different regions. VNTR profiling is a technique used in DNA