NEETZoologyMolecular Basis of Inheritance
Match List I with List II. List I (Step in DNA fingerprinting) List II (Tool / Material used) A. Digestion of DNA I. Autoradiography B. Separation of DNA fragments II. Nitrocellulose or nylon membrane C. Transferring of DNA fragments III. Restriction endonucleases D. Detection of hybridised DNA IV. Gel electrophoresis Choose the correct answer from the options given below:
Options
- AA - III, B - IV, C - II, D - I
- BA - III, B - II, C - IV, D - I
- CA - IV, B - III, C - II, D - I
- DA - III, B - IV, C - I, D - II
Correct answer
A. A - III, B - IV, C - II, D - I
Step-by-step solution
In DNA fingerprinting, the steps and their corresponding tools or materials are as follows: Digestion of DNA is carried out by restriction endonucleases (A - III). Separation of the digested DNA fragments is achieved by gel electrophoresis (B - IV). Transferring (blotting) of the separated DNA fragments is done onto synthetic membranes such as nitrocellulose or nylon (C - II). Detection of the hybridised DNA fragments is performed by autoradiography (D - I). Therefore, the correct matching is A - III, B - IV, C - I