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NEETZoologyMolecular Basis of Inheritance

A bacterial culture is treated with a specific chemical that completely inhibits the binding of the Rho ( ) factor to the RNA polymerase enzyme, while the binding of the Sigma ( ) factor remains unaffected. What will be the most direct and immediate consequence on the transcription process in these bacteria?

Options

  1. AThe RNA polymerase will fail to initiate the process of transcription.
  2. BThe RNA polymerase will fall off prematurely before elongating the transcript.
  3. CThe RNA polymerase will fail to terminate transcription at the correct site.
  4. DThe splicing of the newly synthesized RNA will not occur.

Correct answer

C. The RNA polymerase will fail to terminate transcription at the correct site.

Step-by-step solution

In bacteria, the core RNA polymerase enzyme is capable of catalyzing elongation but requires transient association with specific factors for initiation and termination. The Sigma ( ) factor binds to RNA polymerase to initiate transcription, while the Rho ( ) factor binds to it to terminate the process. Since the chemical only inhibits the Rho factor and leaves the Sigma factor unaffected, initiation and elongation will proceed normally. However, without the Rho factor, the RNA polymerase will be unable to recognize

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