NEETZoologyMolecular Basis of Inheritance
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): In the 'sequence annotation' methodology of the Human Genome Project, the total DNA from a cell is converted into smaller random fragments and cloned in specialized hosts. Reason (R): There are technical limitations in sequencing very long pieces of intact DNA polymers. In the light of the above st
Options
- ABoth (A) and (R) are true and (R) is the correct explanation of (A)
- BBoth (A) and (R) are true but (R) is not the correct explanation of (A)
- C(A) is true but (R) is false
- D(A) is false but (R) is true
Correct answer
A. Both (A) and (R) are true and (R) is the correct explanation of (A)
Step-by-step solution
Both Assertion (A) and Reason (R) are true, and (R) is the correct explanation of (A). Sequence annotation is a blind approach that involves sequencing the whole set of genome (both coding and non-coding sequences). Since DNA is a very long polymer, there are technical limitations in sequencing very long pieces of DNA intact. Therefore, the total DNA from a cell is isolated, converted into random fragments of relatively smaller sizes, and cloned in suitable hosts using specialized vectors (like BAC and YAC) to ampl