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NEETZoologyMolecular Basis of Inheritance

An mRNA molecule has the following nucleotide sequence: 5' - U A C G G A U C - 3' What will be the sequence and polarity of the template DNA strand from which it was transcribed?

Options

  1. A5' - T A C G G A T C - 3'
  2. B5' - A T G C C T A G - 3'
  3. C3' - A T G C C T A G - 5'
  4. D3' - A U G C C U A G - 5'

Correct answer

C. 3' - A T G C C T A G - 5'

Step-by-step solution

The template DNA strand is complementary and antiparallel to the mRNA sequence. Given mRNA: 5' - U A C G G A U C - 3' By applying complementary base pairing rules (U pairs with A, A pairs with T, C pairs with G, G pairs with C) and reversing the polarity, the template DNA strand is: 3' - A T G C C T A G - 5' The sequence 5' - T A C G G A T C - 3' represents the coding strand, not the template strand. The sequence 5' - A T G C C T A G - 3' has the wrong polarity, and DNA does not contain uracil (U). Answer: 3' - A T

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