NEETZoologyMolecular Basis of Inheritance
Match List-I with List-II regarding the methodologies and tools of the Human Genome Project. List-I List-II (A) Expressed Sequence Tags (I) Vectors used for cloning DNA fragments (B) Sequence Annotation (II) Identifying all the genes that are expressed as RNA (C) BAC and YAC (III) Sequencing the whole set of genome containing coding and non-coding sequences (D) Automated DNA sequencers (IV) Work on the principle of a
Options
- A(A)-(III), (B)-(II), (C)-(I), (D)-(IV)
- B(A)-(II), (B)-(III), (C)-(IV), (D)-(I)
- C(A)-(II), (B)-(III), (C)-(I), (D)-(IV)
- D(A)-(III), (B)-(II), (C)-(IV), (D)-(I)
Correct answer
C. (A)-(II), (B)-(III), (C)-(I), (D)-(IV)
Step-by-step solution
According to NCERT, the Human Genome Project involved two major approaches. One approach focused on identifying all the genes that are expressed as RNA, referred to as Expressed Sequence Tags (ESTs). The other took the blind approach of simply sequencing the whole set of genome that contained all the coding and non-coding sequence, and later assigning different regions in the sequence with functions, which is termed Sequence Annotation. For sequencing, the total DNA from a cell is isolated and converted into random