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NEETZoologyMolecular Basis of Inheritance

A researcher is performing DNA fingerprinting in the laboratory. They isolate the DNA, digest it with restriction endonucleases, and separate the fragments on an agarose gel. Immediately after this, they add a radiolabelled VNTR probe directly onto the gel for hybridisation, followed by autoradiography. However, the procedure fails to produce a clear autoradiogram. Which essential step of the standard DNA fingerprint

Options

  1. AAmplification of the DNA using PCR
  2. BDigestion of the DNA by restriction endonucleases
  3. CSeparation of the DNA fragments by gel electrophoresis
  4. DTransferring (blotting) of separated DNA fragments to synthetic membranes

Correct answer

D. Transferring (blotting) of separated DNA fragments to synthetic membranes

Step-by-step solution

According to the standard DNA fingerprinting technique developed by Alec Jeffreys, the correct sequence of steps is: 1. Isolation of DNA 2. Digestion of DNA by restriction endonucleases 3. Separation of DNA fragments by electrophoresis 4. Transferring (blotting) of separated DNA fragments to synthetic membranes, such as nitrocellulose or nylon 5. Hybridisation using a radiolabelled VNTR probe 6. Detection of hybridised DNA fragments by autoradiography In the given scenario, the researcher attempted hybridisation di

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