NEETZoologyMolecular Basis of Inheritance
During the Human Genome Project, two major approaches were used for sequencing. Which of the following correctly describes the methodology known as Expressed Sequence Tags (ESTs)?
Options
- ASequencing the whole set of genome containing all coding and non-coding sequences
- BIdentifying all the genes that are expressed as RNA
- CAssigning genetic markers using restriction endonuclease recognition sites
- DIdentifying differences in specific regions of DNA sequence called variable number of tandem repeats
Correct answer
B. Identifying all the genes that are expressed as RNA
Step-by-step solution
The Human Genome Project utilized two major methodologies. The Expressed Sequence Tags (ESTs) approach focused specifically on identifying all the genes that are expressed as RNA. Sequencing the entire genome (both coding and non-coding regions) and assigning functions to different segments is known as Sequence Annotation. Assigning genetic markers using restriction sites is a part of genetic mapping, while identifying variable number of tandem repeats is the basis of DNA fingerprinting. Answer: Identifying all the