NEET2011ZoologyMolecular Basis of InheritanceActual
Which one of the following options is a correct match of phenomenon and its explanation?
Options
- AReverse Transcription PCR - Many copies of a DNA sequence.
- BCentral dogma - RNA DNA Protein RNA.
- CRNA silencing - Use of ds-RNA to stop the expression of ss-RNA.
- DTranscription - Process of formation of RNA & proteins.
Correct answer
A. Reverse Transcription PCR - Many copies of a DNA sequence.
Step-by-step solution
Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a variant of Polymerase Chain Reaction (PCR), a laboratory technique commonly used in molecular biology to generate many copies of a DNA sequence, a process termed "amplification". In RT-PCR, however, an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or c DNA) using the enzyme reverse transcriptase, and the resulting c DNA is amplified using traditional PCR or Real-Time PCR. Reverse transcription PCR (RT-PCR)is not to b