AIIMS 201826 May
- Exam
- AIIMS
- Year
- 2018
- Date
- 26 May
- Subject
- Zoology
- Chapter
- Strategies for Enhancement in Food Production
AIIMS 2018 · 26 May · Zoology · Strategies for Enhancement in Food Production
Assertion : Hybridoma cells are shifted to a medium deficient in...
Assertion : Hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesised by myeloma cells.
Reason: The nutrient deficient medium allows selection of hybridoma cells.
Options
- AIf both assertion and reason are true and reason is the correct explanation of assertion.
- BIf both assertion and reason are true but reason is not the correct explanation of assertion.
- CIf assertion is true but reason is false.
- DIf both assertion and reason are false.
Correct answer
A. If both assertion and reason are true and reason is the correct explanation of assertion.
Step-by-step solution
The hybridoma cells are shifted to a medium deficient in nutrient which cannot be synthesised by myeloma cells so that myeloma cells could be removed or hybridoma cells could be purified. The unfused myeloma cells die while hybridoma cells survive. The hybridoma cells are allowed to multiply separately.