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AIIMS 2011
Exam
AIIMS
Year
2011
Subject
Zoology
Chapter
Molecular Basis of Inheritance

AIIMS 2011 · Zoology · Molecular Basis of Inheritance

Which one of the following options is a correct match of phenomenon...

Which one of the following options is a correct match of phenomenon and its explanation?

Options

  1. AReverse Transcription PCR - Many copies of a DNA sequence.
  2. BCentral dogma - RNA →\rightarrow DNA →\rightarrow Protein →\rightarrow RNA.
  3. CRNA silencing - Use of ds-RNA to stop the expression of ss-RNA.
  4. DTranscription - Process of formation of RNA \& proteins.

Correct answer

A. Reverse Transcription PCR - Many copies of a DNA sequence.

Step-by-step solution

Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a variant of Polymerase Chain Reaction (PCR), a laboratory technique commonly used in molecular biology to generate many copies of a DNA sequence, a process termed "amplification". In RT-PCR, however, an RNA strand is first reverse transcribed into its DNA complement (complementary DNA, or cc DNA) using the enzyme reverse transcriptase, and the resulting cc DNA is amplified using traditional PCR or Real-Time PCR. Reverse transcription PCR (RT-PCR)is not to be confused with RealTime Polymerase Chain Reaction (Q-PCR/qRTPCR), RNA silencing (also called as posttranscriptional gene silencing PTGS) refers to a family of gene silencing effects by which the expression of one or more genes is down regulated or entirely suppressed by the introduction of an antisense (single stranded) RNA molecule. Transcription is the process of creating a complementary RNA copy of a sequence of DNA.
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