If an enzyme reaction follows the Michaelis Menten kinetics represented by the equation: V₀=V_ [S] K_M+[S] where, V₀ is the initial reaction velocity, V_ is the maximal reaction velocity, K_M is the Michaelis constant and [S] is the substrate concentration. Which of the following statements is correct?

Options

1. AK_M is the concentration of the enzyme at the optimal pH .
2. BK_M is the reaction velocity at half the optimal substrate concentration.
3. CK_M is the concentration of substrate when the velocity of the reaction is half that of the maximal velocity.
4. DK_M is the concentration of the enzyme at the optimal temperature.