KCET2026BiologyMolecular Basis of Inheritance
Arrange the below given steps of DNA fingerprinting in sequence by selecting the appropriate option. a) Digestion of DNA by restriction endonucleases b) Autoradiography c) Blotting of DNA fragments to nitrocellulose membrane d) Isolation of DNA e) Separation of DNA fragments by electrophoresis
Options
- A(a), (e), (d), (c), (b)
- B(e), (a), (d), (b), (c)
- C(d), (a), (e), (c), (b)
- D(c), (b), (a), (e), (d)
Correct answer
C. (d), (a), (e), (c), (b)
Step-by-step solution
The steps of DNA fingerprinting are carried out in the following sequence: 1. Isolation of DNA (d) 2. Digestion of DNA by restriction endonucleases (a) 3. Separation of DNA fragments by electrophoresis (e) 4. Blotting of DNA fragments to synthetic membranes, such as nitrocellulose or nylon (c) 5. Hybridisation using labelled VNTR probe 6. Detection of hybridised DNA fragments by autoradiography (b) The correct sequence is (d), (a), (e), (c), (b). Answer: (d), (a), (e), (c), (b)