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MHT CET Medical202623 April 2026Evening ShiftBiologyBiomolecules (B)Actual

An enzyme catalyzes a reaction at a high rate at 37^ C but becomes inactive at 60^ C. This is because

Options

  1. AThe enzyme is denatured at high temperature.
  2. BThe enzyme is activated only at low temperature
  3. CThe substrate becomes inactive at high temperature.
  4. DThe enzyme-substrate complex becomes more stable

Correct answer

A. The enzyme is denatured at high temperature.

Step-by-step solution

Enzymes are generally proteinaceous in nature and have a specific three-dimensional structure that forms their active site. They work optimally at a specific temperature, which is around 37^ C for most human enzymes. At high temperatures such as 60^ C, the thermal energy disrupts the weak interactions (like hydrogen bonds and hydrophobic interactions) that maintain the enzyme's tertiary structure. This causes the enzyme to unfold and lose its specific shape, a process known as denaturation. Once denatured, the acti

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