MHT CET 202623 AprilEvening Shift
- Exam
- MHT CET
- Year
- 2026
- Date
- 23 April
- Shift
- Evening Shift
- Subject
- Biology
- Chapter
- Biomolecules (B)
MHT CET 2026 · 23 April · Evening Shift · Biology · Biomolecules (B)
An enzyme catalyzes a reaction at a high rate at 37^ ^ C but...
An enzyme catalyzes a reaction at a high rate at 37^{}^{\circ} C but becomes inactive at 60^{}^{\circ} C. This is because
Options
- AThe enzyme is denatured at high temperature.
- BThe enzyme is activated only at low temperature
- CThe substrate becomes inactive at high temperature.
- DThe enzyme-substrate complex becomes more stable
Correct answer
A. The enzyme is denatured at high temperature.
Step-by-step solution
Enzymes are generally proteinaceous in nature and have a specific three-dimensional structure that forms their active site. They work optimally at a specific temperature, which is around C for most human enzymes.
At high temperatures such as C, the thermal energy disrupts the weak interactions (like hydrogen bonds and hydrophobic interactions) that maintain the enzyme's tertiary structure. This causes the enzyme to unfold and lose its specific shape, a process known as denaturation.
Once denatured, the active site is destroyed, and the enzyme can no longer bind to its substrate, rendering it inactive.
Answer: The enzyme is denatured at high temperature.
At high temperatures such as C, the thermal energy disrupts the weak interactions (like hydrogen bonds and hydrophobic interactions) that maintain the enzyme's tertiary structure. This causes the enzyme to unfold and lose its specific shape, a process known as denaturation.
Once denatured, the active site is destroyed, and the enzyme can no longer bind to its substrate, rendering it inactive.
Answer: The enzyme is denatured at high temperature.