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MHT CET Medical202622 April 2026Morning ShiftBiologyBiomolecules (B)Actual

Which one of the following statements is INCORRECT based on the above graph showing the rate of enzyme action?

Options

  1. AK _m or Michaelis-Menten constant is the substrate concentration to produce half of the maximum velocity in an
  2. BIt shows that half of the enzyme molecules are bound with substrate molecules when substate concentration equa
  3. CA high K _m value indicates a strong affinity between enzyme and substrate.
  4. DThe maximum rate of an enzyme reaction is directly proportional to the concentration of enzymes.

Correct answer

C. A high K _m value indicates a strong affinity between enzyme and substrate.

Step-by-step solution

The Michaelis-Menten constant ( K_m ) is defined as the substrate concentration at which the reaction rate is half of its maximum velocity ( V_ max /2 ). This directly supports statement (1). At K_m , the reaction rate is V_ max /2 . This means that exactly half of the active sites on the enzyme molecules are occupied by substrate molecules. This supports statement (2). The maximum rate of an enzyme-catalyzed reaction ( V_ max ) is directly proportional to the total concentration of the enzyme present ( V_ max = k_

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