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Vacuoles: Membrane-bound space found in the cytoplasm is called vacuole. A vacuole contains water, sap, excretory product and other useless materials. The vacuole is bound by a single membrane; called tonoplast. The vacuole can occupy up to 90% of the volume in a plant cell. In plant cells, the tonoplast facilitates the transport of a number of ions and other materials against concentration gradient into the vacuole. Hence, their concentration is significantly higher in the vacuole than in the cytoplasm. In Amoeba