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NEET 2005
Exam
NEET
Year
2005
Subject
Botany
Chapter
Cell - The Unit of Life

NEET 2005 · Botany · Cell - The Unit of Life

A student wishes to study the cell structure under a light...

A student wishes to study the cell structure under a light microscope having 10X10 \mathrm{X} eyepiece and 45X45 \mathrm{X} objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution?

Options

  1. Ablue
  2. Bgreen
  3. Cyellow
  4. Dred

Correct answer

A. blue

Step-by-step solution

In order to increase the resolution, the specimen must be viewed using either shorter wavelength (λ)(\lambda) light or through an imaging medium with a relatively high refractive index or with optical components which have a high numerical aperture (NA). thus, resolution of microscope in inversely proportional to the wavelength of the light used. Δ\Deltad=λ2nsin⁡θ\frac{\lambda}{2 n \sin \theta}Where, λ= wavelength n= refractive index θ= half angle subtended \Deltad= shortest distance. \begin{aligned}& \lambda=\text{ wavelength }\\ & n=\text{ refractive index }\\ & \theta=\text{ half angle subtended }\\ & \Deltad=\text{ shortest distance. } \end{aligned}
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