A student wishes to study the cell structure under a light microscope having 10 X eyepiece and 45 X objective. He should illuminate the object by which one of the following colours of light so as to get the best possible resolution?

Step-by-step solution

In order to increase the resolution, the specimen must be viewed using either shorter wavelength ( ) light or through an imaging medium with a relatively high refractive index or with optical components which have a high numerical aperture (NA). thus, resolution of microscope in inversely proportional to the wavelength of the light used. d= 2 n Where, aligned & = wavelength & n= refractive index & = half angle subtended & d= shortest distance. aligned