In glycolysis, during oxidation electrons are removed by:

When 3-phosphoglyceraldehyde is converted into 1,3 diphosphoglyceric acid, two electrons and two protons are released which are utilised to convert $\mathrm{NAD}^{+}$to $\mathrm{NADH}$. $\mathrm{NAD}^{+}+$2 \mathrm{H}^{+}+2 \mathrme^{-} $\rightarrow \mathrm{NADH}+\mathrm{H}^{+}$Related Theory Glycolysis is a partial breakdown of a sixcarbon glucose molecule into two, threecarbon molecules of pyruvate, $2 \mathrm{NADH}, 2 \mathrm{H}^{+}$, and 2 net ATP as a result of substrate-level phosphorylation. Glycolysis occurs in the cytoplasm of the cell.