Chargaff's rule states that in an organism

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Aamount of adenine (A) is equal to that of thymine $(\mathrm{T})$ and the amount of guanine $(\mathrm{G})$ is equal to that of cytosine (C)
Bamount of adenine (A) is equal to that of guanine (G) and the amount of thymine $(T)$ is equal to that of cytosine (C)
Camount of adenine (A) is equal to that of cytosine $(\mathrm{C})$ and the amount of thymine $(T)$ is equal to that of guanine (G)
Damounts of all bases are equal

A. amount of adenine (A) is equal to that of thymine $(\mathrm{T})$ and the amount of guanine $(\mathrm{G})$ is equal to that of cytosine (C)

Chargaff rule states that in DNA there is always equality in quantity between the bases

\mathrm{A}

and

\mathrm{T}

and between the bases

\mathrm{G}

and

\mathrm{C}

. Related Theory In all the species the molar ratios

[A]=[T]

and

[C]=[G]

, and that ratio

[C+G] /[A+T]

was typically less than the unity with

[C+G]

is less abundant.