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NEET 2022
Exam
NEET
Year
2022
Subject
Zoology
Chapter
Molecular Basis of Inheritance

NEET 2022 · Zoology · Molecular Basis of Inheritance

Ten E. coli cells with N 15 -dsDNA are incubated in medium...

Ten E. coli cells with N15N 15-dsDNA are incubated in medium containing N14N 14 nucleotide. After 6060 minutes, how many E. coli cells will have DNA totally free from N15N 15?

Options

  1. A4040 cells
  2. B6060 cells
  3. C8080 cells
  4. D2020 cells

Correct answer

B. 6060 cells

Step-by-step solution

E. coli has a doubling time of 20 minutes. Thus, in 60 minutes, there would be 3 cycles of doubling. That means, after 60 minutes, there would be 80 E. coli cells, if we start from 10 initial cells.

In the first cycle, all 20 cells would be hybrid DNA containing N15/N14state{N}^{15} / {N}^{14}state. The second cycle would yield some 40 cells and out of those, 20 cells would be totally free from N15{N}^{15} DNA. In the last cycle, there would be 80 cells created and 60 would have N14/N14{N}^{14} / {N}^{14} state and only 20 cells would have N15/N14state{N}^{15} / {N}^{14}state.

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