NEET2003ZoologyMolecular Basis of InheritanceActual
During translation initiation in prokaryotes, a GTP molecule is needed in:
Options
- Aformation of formyl-met- t RNA
- Bbinding of 30 ~S subunit of ribosome with m RNA
- Cassociation of 30 ~S m RNA with formyl-mel- t RNA
- Dassociation of 50 ~S subunit of ribosome with initiation complex
Correct answer
C. association of 30 ~S m RNA with formyl-mel- t RNA
Step-by-step solution
Formyl-met-tRNA is formed by addition of methionine to tRNA fMet (formyl-methionine specifictRNA) by the fMet-tRNA synthetase to produce methioninetRNA and then transfer of formyl group from N10 formyl tetrahydrofolate to the amino group of the Met residue to produce N -formyl methionine tRNA. Binding of 30 ~S ribosomal subunit to two initiation factors, IF-1 and IF-3 initiates translation. Factor IF-3 prevents the 30 ~S and 50 ~S subunits from combining prematurely and mediated binding of mRNA to 30 ~S subunit. Th