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In order to obtain virus-free plants through tissue culture the best method is:

Options

  1. Ameristem culture
  2. Bprotoplast culture
  3. Cembryo rescue
  4. Danther culture

Correct answer

A. meristem culture

Step-by-step solution

The virus infected plants only the meristematic cells of the apical dome are devoid of virus. Therefore, by using shoot apical meristem culture, virus free plants can be raised. Related Theory In the meristem there is no cells differentiation. Virus can't move from cell to cell through plasmodesmesta using their Movement protein. Meristematic cells are considered as virus free because: (1) Meristematic cells have high multiplication rate than viral cell multiplication rates. (2) Viruses generally move in plant body

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