NEST2023BiologyBiomolecules (B)
A biochemist wants to purify a protein X (molecular weight =30.2 kDa and pI =7.5 ) from a solution containing proteins X and Y (molecular weight =30.9 kDa and pI =3.5) using ion exchange chromatography. In this technique, an anion exchange resin is positively charged and a cation exchange resin is negatively charged. The most appropriate resin where protein X , but not Y will remain bound is
Options
- Acation exchanger at pH =7.5 .
- Banion exchanger at pH =2.5 .
- Ccation exchanger at pH =5.0 .
- Danion exchanger at pH =7.5 .
Correct answer
C. cation exchanger at pH =5.0 .
Step-by-step solution
No solution available.