Following graphs show the effect of change in concentration of substrate on enzyme activity: Graph - A: Graph - B: Graph - C: Graph - D: In which of the above curve, the enzyme shows maximum substrate affinity?

Step-by-step solution

K m indicates the affinity of the enzyme towards its substrate. Km = 1 2 V m a x K m value differs from substrate to substrate because different enzymes differ in their affinity towards different substrates. A high K m indicates low affinity, whereas a low K m shows strong affinity. Out of the given graphs, K m value is lowest in this option and hence shows maximum substrate affinity.