NEETBotanyPhotosynthesis in Higher Plants
Identify the correct statement regarding the spatial arrangement of complexes and proton accumulation in the thylakoid membrane during light reactions.
Options
- AThe water splitting complex is physically located on the outer side of the thylakoid membrane.
- BThe NADP reductase enzyme is located on the stroma side of the thylakoid membrane.
- CDuring electron transport, protons accumulate in the stroma of the chloroplast.
- DThe primary electron acceptor of PS II is located towards the inner side of the thylakoid membrane.
Correct answer
B. The NADP reductase enzyme is located on the stroma side of the thylakoid membrane.
Step-by-step solution
According to the chemiosmotic hypothesis in chloroplasts: The NADP reductase enzyme is located on the stroma side of the membrane. Along with electrons from the acceptor of PS I, protons are necessary for the reduction of NADP ^+ to NADPH + H ^+ . These protons are removed from the stroma. Thus, this statement is correct. The water splitting complex is associated with PS II, which is physically located on the inner side of the membrane (lumen side), not the outer side. During electron transport, protons accumulate