NEETZoologyBiotechnology and Its Applications
What is the purpose of using a radioactive molecule (probe) in diagnosing diseases through PCR?
Options
- ATo detect the presence of pathogen
- BTo detect mutated genes
- CTo detect antigens in ELISA
- DTo detect antibodies synthesized against pathogen
Correct answer
B. To detect mutated genes
Step-by-step solution
You know that for effective treatment of a disease, early diagnosis and understanding its pathophysiology is very important. Using conventional methods of diagnosis (serum and urine analysis, etc.) early detection is not possible. Recombinant DNA technology, Polymerase Chain Reaction (PCR) and Enzyme Linked Immuno-sorbent Assay (ELISA) are some of the techniques that serve the purpose of early diagnosis. Presence of a pathogen (bacteria, viruses, etc.) is normally suspected only when the pathogen has produced a dis