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NEET Biotechnology and Its Applications PYQs with Solutions

440 previous year questions from Biotechnology and Its Applications (NEET UG Zoology) with answers and solutions. Numbered list, year tags, and one-tap solutions — built for serious JEE / NEET practice.

440 questionsZoology2002–2026Answers + solutions

About Biotechnology and Its Applications PYQs

Biotechnology and Its Applications has 440 previous year questions in the Quantrex Academy NEET UG Zoology bank. The 220 questions in this list come from NEET (208) and JIPMER (12), spanning 2002 to 2026. Each question opens on its own page with the options, the correct answer and the worked solution where available.

Questions by year (this list)

  • 2026: 3
  • 2025: 3
  • 2024: 5
  • 2023: 3
  • 2022: 5
  • 2021: 5
  • 2020: 5
  • 2019: 4
  • 2018: 3
  • 2016: 4
  • 2015: 5
  • 2014: 4

Biotechnology and Its Applications questions with solutions

1

The inactive form of Bt toxin is converted to the active form in the insect gut __________.

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2

The human protein named α - 1 -antitrypsin, obtained from transgenic animals, is used for the treatment of :

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3

The toxin proteins, isolated from Bacillus thuringiensis , coded by which of the following genes would control cotton bollworms and corn borer, respectively ?

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4

Given below are two statements : Statement I : Transfer RNAs and ribosomal RNA do not interact with mRNA. Statement II : RNA interference (RNAi) takes place in all eukaryotic

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5

Silencing of specific mRNA is possible via RNAi because of

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6

Which of the following genetically engineered organisms was used by Eli Lilly to prepare human insulin?

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7

Match List I with List II : Choose the correct answer form the options given below:

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8

The B t toxin in genetically engineered B t cotton kills the pest by:

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9

Given below are two statements : Statement I : RNA interference takes place in all Eukaryotic organisms as method of cellular defense. Statement II : RNAi involves the silencing

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10

Given below are two statements: Statement I : Bt toxins are insect group specific and coded by a gene cry IAc. Statement II : Bt toxin exists as inactive protoxin in B.

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11

Match List-I with List-II : List-I List-II A.Genetically engineered Human Insulin I. Gene therapy B. GM Cotton II. E. coli C. ADA Deficiency III. Antigen-antibody interaction D.

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12

Which one of the following techniques does not serve the purpose of early diagnosis of a disease for its early treatment?

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13

Match List - I with List - II. Choose the correct answer from the options given below :

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14

Select incorrect statement, regarding chemical structure of insulin.

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15

Milk of transgenic 'Cow Rosie' was nutritionally more balanced product for human babies than natural cow milk because it contained:

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16

Breedings crops with higher levels of vitamins and minerals or higher proteins and healthier fats is called:

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17

Transposons can be used during which one of the following?

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18

In gene therapy of adenosine deaminase (ADA) deficiency, the patient requires a periodic infusion of genetically engineered lymphocytes because:

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19

Statements related to human Insulin are given below: Which statement(s) is/are correct about genetically engineered Insulin? (a) Pro-hormone insulin contain extra stretch of

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20

With regard to insulin choose correct options. (a) C-peptide is not present in mature insulin. (b) The insulin produced by rDNA technology has C-peptide. (c) The pro-insulin has

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21

For effective treatment of the disease, early diagnosis and understanding its pathophysiology is very important. Which of the following molecular diagnostic techniques is very

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22

When gene targeting involving gene amplification is attempted in an individual's tissue to treat disease, it is known as:

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23

Now a days it is possible to detect the mutated gene causing cancer by allowing radioactive probe to hybridise its complimentary DNA in a clone of cells, followed by its detection

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24

The Adenosine deaminase deficiency results into:

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25

RNA interference is used for which of the following purposes in the field of biotechnology?

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26

The laws and rules to prevent unauthorised exploitation of bioresources are termed as:

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27

Which of the following statements is not correct?

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28

Bt cotton variety that was developed by the introduction of toxin gene of Bacillus thuringiensis (Bt) is resistant to

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29

Match the following columns and select the correct option. Column-I Column-II (a) Bt cotton (i) Gene therapy (b) Adenosine deaminase deficiency (ii) Cellular defence (c) RNAi

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30

Exploitation of bioresources of a nation by multinational companies without authorisation from the concerned country is referred to as

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31

In RNA i , the genes are silenced using

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32

Which of the following is true for Golden rice?

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33

What triggers activation of protoxin to active Bt toxin of Bacillus thuringiensis in a boll worm?

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34

A 'new' variety of rice was patented by a foreign company, though such varieties have been present in India for a long time. This is related to

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35

Use of bioresources by multinational companies and organizations without authorisation from the concerned country and its people is called

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36

In India, the organisation responsible for assessing the safety of introducing genetically modified organisms for public use is:

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37

Golden ages of reptiles and fishes are respectively

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38

The two polypeptides of human insulin are linked together by:

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39

Which kind of therapy was given in 1990 to a four-year-old girl with adenosine deaminase (ADA) deficiency?

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40

Which part of the tobacco plant is infected by Meloidogyne incognita ?

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41

Who received Nobel Prize in 1951 for the development of vaccine for yellow fever?

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42

Golden rice is a genetically modified crop plant where the incorporated gene is meant for the biosynthesis of

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43

In Bt cotton, the Bt toxin present in plant tissue as pro-toxin is converted into active toxin due to:

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44

The crops engineered with Bt-toxin are resistant/tolerant to:

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45

Which body of the Government of India regulates GM research and safety of introducing GM organisms for public services?

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46

The protein α-1 antitrypsin is used to treat which of the following disease?

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47

Bacillus thuringiensis (B t) strains have been used for designing novel

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48

The first human hormone produced by recombinant DNA technology is:

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49

An analysis of chromosomal DNA using the Southern hybrization technique does not use:

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50

Herbicide resistant gene in plant is

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51

Which one of the following vectors is used to replace the defective gene in gene therapy?

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52

RNA interference involves:

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53

Satellite RNAs are present in some:

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54

Which of the following has maximum genetic diversity in India?

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55

Which of the following B t crops is being grown in India by the farmers?

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56

Product of biotechnology is

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57

Which of the following bio-engineered bacteria is utilized for cleaning of marine oil slicks?

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58

A transgenic food crop, which may help in solving the problem of nightblindness in developing countries is

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59

Consumption of which one of the following foods can prevent the kind of blindness associated with vitamin-A deficiency?

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60

Tobacco plants resistant to a nematode have been developed by the introduction of DNA that produced (in the host cells)

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61

The first clinical gene therapy was given for treating

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62

Which one of the following is an example of carrying out blological control of pests/diseases using microbes?

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63

Nucleic acid segment tagged with a radiactive molecule is called

JIPMER 2011 Solution
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64

'Jaya' and 'Ratna' developed for green revolution in India are the varieties of

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65

Which of the following enzymes carries out the initial step in the digestion of milk in humans?

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66

Maximum number of existing transgenic animals is of

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67

'Himgiri' developed by hybridisation and selection for disease resistance against rust pathogens is a variety of

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68

Bacillus thuringiensis forms protein crystals, which contain insecticidal protein

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69

Which one of the following techniques made it possible to genetically engineer living organism?

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70

The process of RNA interference has been used in the development of plants resistant to

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71

Read the following statement having two blanks ( 1 and 2 ). A drug used for (1) patients is obtained from a species of the organism (2) ...... The one correct option for the two

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72

An organism used as a biofertilizer for raising soyabean crop is

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73

Consider the following statements (I-IV) about organic farming. I. Utilizes genetically modified crops like B t cotton II. Uses only naturally produced inputs like compost III.

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74

Silencing of mRNA has been used in producing transgenic plants resistant to

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75

The genetically-modified (GM) brinjal in India has been developed for

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76

A common biocontrol agent for the control of plant diseases is

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77

Some of the characteristics of B t cotton are

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78

Which one of the following is not used in organic farming?

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79

An improved variety of transgenic basmati rice

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80

Which one of the following cannot be used for preparation of vaccines against plague?

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81

Which one of the following is now being commercially produced by biotechnological procedures?

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82

Black (stem) rust of wheat is caused by

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83

The bacterium Bacillus thuringiensis is widely used in contemporary biology as a/an

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84

Which of the following is not used as a biopesticide?

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85

The bacterium Bacillus thuringiensis is widely used in contemporary biology as -

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86

Transgenic plants are

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87

What is true about Bt toxin?

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88

The genetic defect-adenosine deaminase (ADA) deficiency may be cured permanently by

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89

What is true about B t toxin?

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90

Which one of the following is commonly used in transfer of foreign DNA into crop plants?

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91

A transgenic food crop, which may help in solving the problem of nightblindness in developing countries is

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92

Consider the following four measures (A-D) that could be taken to successfully grow chick pea in an area where bacterial blight disease is common (A) spray with Bordeaux mixture

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93

Main objective of production/use of herbicide resistant GM crops is to

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94

Human insulin is being commercially produced from a transgenic species of

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95

Cry-I endotoxins obtained from Bacillus thuringiensis are effective against

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96

Which one of the following proved effective for biological control of nematodal diseases in plants?

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97

A transgeneic food crop which may help in solving the problem of night blindness in developing countries is

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98

Cry 1 endotoxins obtained from Bacillus Thuringiensis are effective against

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99

Which one of the following proved effective for biological control of nematodal disease in plants?

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100

Human insulin is being commercially produced from a transgenic species of

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101

What is antisense technology?

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102

Golden rice is a transgenic crop of the future with the following improved trait

JIPMER 2007 Solution
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103

Bacillus thuringiensis (Bt) strains have been used for designing novel

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104

Golden rice is a promising transgenic crop. When released for cultivation, it will help in:

NEET 2006 Solution
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105

Which one of the following can help in the diagnosis of a genetical disorder?

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106

Which of the following is generally used lor induced mutagenesis in crop plants?

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107

Using imprints from a plate with complete medium and carrying bacterial colonies, you can select streptomycin resistant mutants and prove that such mutations do not originate as

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108

The name of Norman Borlaug is associated with:

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109

For retting of jute the fermenting microbe used is:

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110

The salivary gland chromosomes in the dipteran larvae, are useful in gene mapping because:

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111

Golden rice is a transgenic crop of the future with the following improved trail:

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112

Bacillus thuringiensis (Bt) strains have been used for designing novel:

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113

The Ti plasmid, is often used for making transgenic plants. The plasmid is found in:

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114

The most likely reason for the development of resistance against pesticides in insects damaging a crop is:

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115

The most thoroughly studied of the known bacteria-plant interactions is the:

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116

Which one of the following bacteria has found extensive use in genetic engineering work in plants?

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117

Maximum application of animal cell culture technology today is in the production of:

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118

Which fungal disease spreads by seed and flowers?

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119

Which of the following crops have been brought to India from New World?

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120

Nucleus of a donor embryonal cell/ somatic cell is transferred to an enucleated egg cell. Then, after the formation of organism what will be true?

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