Read the following statements and choose the correct option: 1. The main challenge for production of insulin using rDNA techniques was getting insulin assembled into an immature form. 2. In 1983, Eli Lily an American company prepared two DNA sequences corresponding to A and B, chains of human insulin and introduced them in E.coli to produce separately, chains of human insulin and introduced them in plasmids of E.coli

Step-by-step solution

In mammals, including humans, insulin is synthesised as a pro-hormone (like a pro-enzyme, the pro-hormone also needs to be processed before it becomes a fully mature and functional hormone) which contains an extra stretch called the C peptide. This C peptide is not present in the mature insulin and is removed during maturation into insulin.The main challenge for production of insulin using rDNA techniques was getting insulin assembled into an mature form. In 1983, Eli Lily an American company prepared two DNA seque