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NEETZoologyBiotechnology and Its Applications

Components of Genetically produced Insulin Function A. Plasmid 1. Cuts DNA at specific sites B. Restriction enzyme 2. Vehicle for gene transfer C. Ligase enzyme 3. Joins DNA fragments D. Host cell (E. coli) 4. Produces the insulin protein

Options

  1. AA - 1, B - 4, C - 3, D - 2
  2. BA - 4, B - 1, C - 2, D - 3
  3. CA - 2, B - 1, C - 3, D - 4
  4. DA - 3, B - 2, C - 4, D - 1

Correct answer

C. A - 2, B - 1, C - 3, D - 4

Step-by-step solution

The plasmid acts as a vehicle for gene transfer (A - 2). Restriction enzymes cut DNA at specific sites (B - 1). Ligase enzymes join DNA fragments (C - 3). The host cell, usually E. coli, produces the insulin protein (D - 4).

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