NEETZoologyBiotechnology and Its Applications
Steps in Insulin Production Description A. Isolation of insulin gene 1. Insertion of gene into a vector B. Cloning of gene into plasmid 2. Separation of insulin from bacterial proteins C. Expression of insulin gene 3. Extraction of gene from human DNA D. Purification of insulin 4. Production of insulin in bacterial cells
Options
- AA - 3, B - 1, C - 4, D - 2
- BA - 1, B - 2, C - 4, D - 3
- CA - 2, B - 4, C - 3, D - 1
- DA - 4, B - 3, C - 1, D - 2
Correct answer
A. A - 3, B - 1, C - 4, D - 2
Step-by-step solution
The insulin gene is first isolated from human DNA (A - 3). This gene is then cloned into a plasmid vector (B - 1). The recombinant plasmid is introduced into bacterial cells where the insulin gene is expressed (C - 4). Finally, the insulin is purified from the bacterial proteins (D - 2).