NEETZoologyBiotechnology and Its Applications
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): The main challenge for the production of insulin using rDNA techniques was getting insulin assembled into a mature form. Reason (R): Prokaryotic host cells like E. coli lack the specific enzymes required to process pro-insulin into mature insulin by removing the C-peptide. In the light of the above
Options
- ABoth (A) and (R) are correct but (R) is not the correct explanation of (A)
- BBoth (A) and (R) are correct and (R) is the correct explanation of (A)
- C(A) is correct but (R) is not correct
- D(A) is not correct but (R) is correct
Correct answer
B. Both (A) and (R) are correct and (R) is the correct explanation of (A)
Step-by-step solution
Assertion (A) is a correct statement. The main challenge in producing recombinant human insulin was assembling it into its mature, active form because insulin is naturally synthesized as an inactive pro-hormone containing an extra C-peptide. Reason (R) is also a correct statement and correctly explains (A). Prokaryotes such as E. coli do not possess the post-translational modification machinery (enzymes) needed to cleave the C-peptide from pro-insulin and form the necessary disulphide bridges. This is why getting t