NEETZoologyBiotechnology and Its Applications
Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): In 1983, the American company Eli Lilly prepared a single DNA sequence corresponding to human proinsulin and introduced it into plasmids of Escherichia coli. Reason (R): Proinsulin contains an extra stretch called C-peptide, which must be removed to form mature insulin, a modification that bacteria
Options
- ABoth (A) and (R) are true and (R) is the correct explanation of (A)
- BBoth (A) and (R) are true but (R) is not the correct explanation of (A)
- C(A) is true but (R) is false
- D(A) is false but (R) is true
Correct answer
D. (A) is false but (R) is true
Step-by-step solution
Assertion (A) is false. In 1983, Eli Lilly did not use a single DNA sequence for proinsulin. Instead, they prepared two separate DNA sequences corresponding to the A and B chains of human insulin and introduced them into plasmids of Escherichia coli to produce the chains separately. Reason (R) is true. Proinsulin contains an extra stretch called the C-peptide. For insulin to become mature and fully active, this C-peptide must be removed. Bacterial cells like Escherichia coli lack the eukaryotic post-translational m