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NEETZoologyBiotechnology and Its Applications

Given below are two statements: one is labelled as Assertion (A) and the other is labelled as Reason (R). Assertion (A): During molecular diagnosis, a clone of cells having a mutated gene does not appear on the photographic film after autoradiography. Reason (R): The radioactive probe used for detection is a double-stranded DNA that lacks complementarity with the mutated gene. In the light of the above statements, ch

Options

  1. ABoth Assertion (A) and Reason (R) are correct and Reason (R) is the correct explanation of Assertion (A)
  2. BBoth Assertion (A) and Reason (R) are correct but Reason (R) is not the correct explanation of Assertion (A)
  3. CAssertion (A) is correct but Reason (R) is not correct
  4. DAssertion (A) is not correct but Reason (R) is correct

Correct answer

C. Assertion (A) is correct but Reason (R) is not correct

Step-by-step solution

The Assertion (A) is correct because the clone having the mutated gene will not appear on the photographic film during autoradiography. This happens because the standard probe does not have complementarity with the mutated gene. The Reason (R) is incorrect because a probe is defined as a single-stranded DNA or RNA tagged with a radioactive molecule, not a double-stranded DNA. For hybridization to occur, the probe must be single-stranded to pair with its complementary sequence. Answer: Assertion (A) is correct but R

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