NEETZoologyBiotechnology and Its Applications
In a biotechnology experiment, an Agrobacterium vector is used to introduce nematode-specific genes into a host plant to develop pest resistance. To successfully initiate RNA interference (RNAi) in the host cells, the introduced DNA must be designed to produce:
Options
- AOnly antisense RNA
- BBoth sense and antisense RNA
- COnly sense RNA
- DA specific toxic protein
Correct answer
B. Both sense and antisense RNA
Step-by-step solution
To develop nematode resistance in plants using RNA interference (RNAi), nematode-specific genes are introduced into the host plant using Agrobacterium vectors. The introduction of DNA is designed such that it produces both sense and antisense RNA in the host cells. These two RNAs are complementary to each other and form a double-stranded RNA (dsRNA) that initiates RNAi. This dsRNA then binds to and prevents the translation of the specific mRNA of the nematode. Producing only sense or only antisense RNA would not fo